Protein engineering services
Protein engineering CRO services including deep mutational scanning, affinity maturation, directed evolution, and AI-driven enzyme optimization for biopharma and industrial biotech
Start a project →Protein engineering approaches for every stage of development
Directed Evolution
Iterative rounds of mutagenesis and selection to improve protein function. We construct random or focused mutagenesis libraries, screen via display or functional assay, and advance the best performers into the next round.
Affinity Maturation
Take a confirmed binder and engineer it for tighter binding. Focused mutagenesis libraries around the binding interface, screened under increasingly stringent conditions. Starting from micromolar affinity, we routinely achieve 10- to 100-fold improvements across 2-3 rounds.
Deep Mutational Scanning
Systematically measure the effect of every possible single amino acid substitution across your protein. The output is a complete fitness landscape showing how each mutation affects binding, expression, stability, or enzymatic activity. Also applied to drug target biology, mapping how mutations affect drug binding and resistance.
Enzyme Optimization
Begins with computational assessment of the enzyme structure to identify positions most likely to improve performance. Structure-guided predictions narrow the mutational search space before wet-lab screening. Computationally prioritized variants are synthesized and screened using custom functional assays.
How a protein engineering project runs at Ranomics
Every engagement follows the same disciplined process. Timeline and scope vary by project complexity, but the structure remains consistent.
Scoping and design
Technical consultation to define the target protein, mutagenesis strategy, screening assay, and success criteria. SOW delivered within 5 business days.
Library construction
Variant library generated via site-saturation mutagenesis, error-prone PCR, or gene synthesis. Library quality verified by NGS before screening.
Screening and selection
Display-based screening (yeast or mammalian) with FACS/MACS selection, or custom functional assay. Multiple rounds for directed evolution campaigns.
Analysis and delivery
NGS quantification, enrichment analysis, fitness scoring, and ranked candidate list. Full technical report with methods, data, and recommendations.
Protein engineering questions
What is deep mutational scanning? +
Deep mutational scanning (DMS) is a high-throughput method that systematically measures the effect of thousands of protein variants on function, stability, or expression in a single experiment. Variants are introduced combinatorially, screened via display or functional assay, and quantified by next-generation sequencing. The output is a comprehensive fitness landscape for your protein.
How many variants can you screen in a single DMS experiment? +
A typical DMS experiment at Ranomics covers 2,000 to 20,000 single-site variants across the target protein. Combinatorial libraries for multi-site scanning can exceed 100,000 variants, depending on the region of interest and assay throughput.
What is the difference between directed evolution and deep mutational scanning? +
Directed evolution iteratively selects for improved function across multiple rounds of mutagenesis and screening. DMS is a single-round, comprehensive measurement of variant effects across the protein. DMS provides a fitness landscape; directed evolution traverses it. They are complementary: DMS data often guides the design of subsequent evolution campaigns.
Can you engineer enzymes for industrial applications? +
Yes. Ranomics has optimized enzymes for thermostability, catalytic activity, substrate specificity, and expression yield using directed evolution and DMS. We work with industrial biotech clients on food-grade enzymes, biocatalysts, and biosynthetic pathway components.
What deliverables do I receive from a protein engineering project? +
Deliverables include a ranked variant list with fitness scores, full NGS enrichment data, sequence files for top candidates, and a technical report summarizing experimental methods, results, and recommendations for follow-up. Raw sequencing data is available upon request.
Start your protein engineering project
Tell us about your protein, the property you want to optimize, and your timeline. We will design a program and send a proposal within 5 business days.
Start a project →