Binder Developability Scout
Score any antibody, nanobody, or designed binder across five developability dimensions (humanness, liability motifs, charge, hydrophobicity, aggregation) before committing designs to synthesis.
Free to use. No account and no credit card required.
Built for antibody engineers checking a candidate before expression, de novo designers scoring an RFdiffusion or BindCraft design before synthesis, and groups preparing humanized Mabs for clinical candidacy.
From sequence to developability report in minutes
Paste a sequence
Input one VH, VL, scFv, VHH, or de novo binder of 10 to 2000 residues as FASTA or plain text. One sequence per run.
Five-dimension scoring
Humanness, liability motifs, charge and pI, hydrophobic patches, and aggregation propensity combined into a 0-1 composite.
Annotated results
A risk tier on the composite, one card per dimension with the numbers behind its subscore, and the full sequence with flagged residues highlighted and annotated on hover.
Fix and re-run
Work through the flagged motifs and residue positions, then score the revised sequence as a fresh run from the link on the results page.
Five-dimension composite developability score
Each binder is scored on a 0-1 scale combining five dimensions drawn from published antibody developability literature and industry CMC practice. Higher scores indicate molecules more likely to survive downstream manufacturing, formulation, and clinical development.
Humanness
Seven-mer overlap against a built-in pool of representative human germline V-regions (5 IGHV, 3 IGKV, 3 IGLV), with the best-matching allele named in the report. Lower deviation from human germline reduces immunogenicity risk and simplifies humanization.
Liability motifs
Sequence-level scan for deamidation (NG, NS), isomerization (DG, DS), N-linked glycosylation (NXS/T), methionine oxidation, and unpaired free cysteines. Each motif flagged with position and CDR context.
Charge and pI
Theoretical isoelectric point and net charge at pH 7.4, scored against published tolerance ranges for clinical Mabs. An extreme pI predicts poor solubility and heightened viscosity at formulation concentrations.
Hydrophobic patches
Kyte-Doolittle hydropathy over an 11-residue sliding window, with windows above +1.5 mean hydropathy counted as patches. Hydrophobic patches drive non-specific binding, poor HIC retention, and elevated aggregation risk.
Aggregation propensity
Per-residue aggregation propensity from an Aggrescan-style a3v scale, with aggregation-prone region (APR) detection. Flags runs of five or more consecutive residues of positive smoothed propensity whose mean across the run reaches 0.3, the stretches associated with self-association.
Catch liabilities before they become attrition
Most developability failures are visible in the sequence long before a molecule reaches a stability study. A deamidation hotspot in CDR-H2, a glycosylation site at a CDR tip, or an extreme theoretical pI: all correctable if caught early, costly if caught at scale.
Developability Scout surfaces these liabilities in a consistent, literature-backed report so every candidate in your campaign is triaged the same way. Use it between design and synthesis, between screening and lead nomination, or on any binder you are considering putting into a wet-lab pipeline.
After running RFdiffusion or BindCraft, before committing de novo designs to gene synthesis
Triaging an in-house antibody for CMC-blocking liabilities before expression, one sequence per run
Evaluating a humanized Mab variable region before freezing the sequence for clinical candidacy
Checking a naive yeast display hit for developability before progressing to affinity maturation
Scoring a revised sequence after editing out a flagged liability motif
From triage to developable, validated binders
Developability Scout tells you where the liabilities are. The next step is either redesigning in, engineering out, or running the triage yourself.
Design binders that pass developability from the start
The AI Binder Sprint runs RFdiffusion, BindCraft, and Boltzgen with developability filters applied in-loop. Campaign outputs are pre-scored and ranked so lead selection is driven by both affinity and developability.
See the AI Binder Sprint → Engineering servicesRescue liability-laden binders
Bring an existing binder with developability concerns. We combine affinity maturation with liability removal using yeast display and deep mutational scanning to preserve function while improving CMC behavior.
See Protein Engineering → Free guideRun the triage yourself
Developability Assessment for De Novo Designed Binders is a free technical guide covering the sequence liability classes, where a sequence scan runs out, a fix-discard-watch workflow to run before ordering DNA, and the open-source tooling with its licences.
Download the guide →Start scoring your binders
Developability Scout is free and needs no account. Paste one sequence and get a scored report. Or talk to us directly about binder design and developability engineering.