Binder Developability Scout
Score any antibody, nanobody, or designed binder across five developability dimensions (humanness, liability motifs, charge, hydrophobicity, aggregation) before committing designs to synthesis.
Free to use. No credit card required. Sign up and start scoring.
Built for antibody engineers triaging panels, de novo designers filtering RFdiffusion and BindCraft outputs, and groups preparing humanized Mabs for clinical candidacy.
From sequence to developability report in minutes
Paste a sequence
Input a VH/VL pair, scFv, VHH, or de novo binder as FASTA or plain text. Multiple sequences scored in a single batch.
Five-dimension scoring
Humanness, liability motifs, charge and pI, hydrophobic patches, and aggregation propensity combined into a 0-1 composite.
Annotated results
Per-residue heatmap of liability hotspots, CDR-level breakdown, and side-by-side comparison across a panel.
Export for your team
Download a CSV of scores and annotated residues, or a PDF report ready to drop into a design review.
Five-dimension composite developability score
Each binder is scored on a 0-1 scale combining five dimensions drawn from published antibody developability literature and industry CMC practice. Higher scores indicate molecules more likely to survive downstream manufacturing, formulation, and clinical development.
Humanness
Germline similarity against the IMGT reference and repertoire frequency against OAS. Lower deviations from observed human antibodies reduce immunogenicity risk and simplify humanization.
Liability motifs
Sequence-level scan for deamidation (NG, NS), isomerization (DG, DS), N-linked glycosylation (NXS/T), methionine oxidation, and unpaired free cysteines. Each motif flagged with position and CDR context.
Charge and pI
Theoretical isoelectric point, net charge at physiological pH, and VH/VL charge asymmetry. Extreme pI or mismatched VH/VL charges predict poor solubility and heightened viscosity at formulation concentrations.
Hydrophobic patches
Kyte-Doolittle sliding-window hydrophobicity with CDR-specific weighting. Surface-exposed hydrophobic patches drive non-specific binding, poor HIC retention, and elevated aggregation risk.
Aggregation propensity
Per-residue aggregation propensity from published hydrophobicity-weighted scales, with aggregation-prone region (APR) detection. Flags contiguous hydrophobic and beta-sheet-prone stretches associated with self-association.
Catch liabilities before they become attrition
Most developability failures are visible in the sequence long before a molecule reaches a stability study. A deamidation hotspot in CDR-H2, a glycosylation site at a CDR tip, or extreme pI mismatch between chains: all correctable if caught early, costly if caught at scale.
Developability Scout surfaces these liabilities in a consistent, literature-backed report so every candidate in your campaign is triaged the same way. Use it between design and synthesis, between screening and lead nomination, or on any binder you are considering putting into a wet-lab pipeline.
After running RFdiffusion or BindCraft, before committing de novo designs to gene synthesis
Triaging an in-house antibody panel for CMC-blocking liabilities before expression
Evaluating a humanized Mab before freezing the sequence for clinical candidacy
Checking naive yeast display hits for developability before progressing to affinity maturation
Comparing VH/VL pairings and CDR variants from campaign outputs on a single scorecard
From triage to developable, validated binders
Developability Scout tells you where the liabilities are. The next step is either redesigning in or engineering out. Two entry points depending on whether you are starting a campaign or rescuing one.
Design binders that pass developability from the start
The AI Binder Sprint runs RFdiffusion, BindCraft, and Boltzgen with developability filters applied in-loop. Campaign outputs are pre-scored and ranked so lead selection is driven by both affinity and developability.
See the AI Binder Sprint → Engineering servicesRescue liability-laden binders
Bring an existing binder with developability concerns. We combine affinity maturation with liability removal using yeast display and deep mutational scanning to preserve function while improving CMC behavior.
See Protein Engineering →Start scoring your binders
Developability Scout is free. Sign up, paste a sequence, and get a scored report in seconds. Or talk to us directly about binder design and developability engineering.