The Biologics Playbook
A practical guide to cell display technologies for protein engineering, covering yeast display, mammalian display, phage display, and the selection strategies that determine whether your campaign produces leads or noise.
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Everything your team needs to choose the right display platform
Yeast surface display
Protocols, library design principles, FACS vs. MACS selection, and when yeast display is the right choice for your target.
Mammalian display
When post-translational modifications matter. HEK293 display systems, library delivery via lentivirus or Cas9 integration, and expression considerations.
Phage display
The workhorse of library screening. Library formats, panning strategies, and comparison to cell-based display methods.
Selection strategy design
How to design selection experiments that produce real leads. Stringency, counter-selection, and avoiding enrichment artifacts.
Decision framework
A practical matrix for choosing the right display platform based on your target, binder format, and downstream application.
Library design
Diversity calculations, format selection, quality control metrics, and how library design determines campaign success.
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